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Journal of the Institute of Brewing , 80—86 The distinct genotypes were examined to determine whether grain with more aleurone might display increased enzyme activity during germination. Strangely, it has also remained unclear whether intraspecific differences in barley aleurone development are of any physiological importance. A Schematic representation of barley grain sectioning prior to microscopy. Gibberellins - Their physiological role. Evolution and development of cell walls in cereal grains. For the germination assay, plates were removed and scored visually to determine the frequency of grain germination at each time point.